Study on relationship between expression of cyclooxygenase-2 in astrocytic tumors and its pathology classification

OBJECTIVE:To detect the expression of cyclooxygenase-2 in astrocytic tumours and analyze the relationship between it and pathology classification of astrocytic tumours.METHODS:Immunohistochemistry technique was adopted to examine the levels of expression of cyclooxygenase-2 protein in astrocytic tumours tissues and non-tumor tissues and the system of image analysis was adopted to detect the results.RESULTS:The positive rates of cyclooxygenase-2 protein in each group were:non-tumor tissues 28.6%(2/7),astrocytic tumours 80.0%(36/45),pilocytic astrocytoma 60.0%(6/10),diffuse astrocytoma 76.9%(10/13),anaplastic astrocytoma 90.0%(9/10),glioblastoma 91.7%(11/12);the expressions of cyclooxygenase-2 protein in each group by immunohistochemical staining(IODA)were:non-tumor tissues(2.18±0.94),astrocytic tumours(6.38±3.68),pilocytic astrocytoma(3.54±1.29),diffuse astrocytoma(5.58±2.78),anaplastic astrocytoma(6.88±2.70),glioblastoma(9.19±4.62).The expression of cyclooxygenase-2 in astrocytic tumours was significantly highter than that in non-tumor tissues(P=0.017).CONCLUSIONS:The over-expression of cyclooxygenase-2 has a close relation with the occurrence of astrocytic tumors.In biology and morphology,the change of gene of pilocytic astrocytoma is different from other astrocytic tumours.The change of expression of cyclooxygenase-2 provides a new way for clinical diagnosis,treatment and prognosis judgment of astrocytic tumours.