Loss of heterozygosity analysis of micro-dissected nasopharyngeal carcinoma tissues at chromosome 16

Objective: To analyze the loss of heterozygosity (LOH) of chromosome 16 in nasopharyngeal carcinoma (NPC). Methods: Tumor tissues were obtained with microdissetion.PCR was used to analyse 38 NPC samples using eight primers on chromosome 16. Results: All of 38 cases were showed with LOH in at least one of the loci analyzed.Among these primers, D16S533 had the highest frequency of LOH, which was 86.1 % (31/33). High frequent LOH ( 50% ) occured at three other loci including D16S398, D16S390 and D16S420, the presentages were 78.8% (26/33 ). 69.4% (25/33 ) and 57.6 (19/33 ). respectively. Conclusions: From this investigation, we found high frequent LOH occurred at chromosome 16p 12. 3, 16q12. 2 and 16q21-22.1I for the first time. These results indicate that more than one (TSGs) related to NPC may harbored at these regions.