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[Study of loss of heterozygosity at chromosome 11q13 in nasopharyngeal carcinoma].

Ying Guo, Yuda Fang, Bi‐Jun Huang

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Abstract

OBJECTIVE: To analyze the loss of heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC). METHODS: Relatively pure tumor tissues were obtained by microdissetion. PCR was used to perform microsatellite analysis of NPC samples using D11S4946, PYGM, D11S449 and INT-2 as primers, which span the MEN-1 gene. RESULTS: Thirty-six of 38 cases studied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (78.8%). The LOH frequency was 51.5% for INT-2, 45.5% for PYGM and 45.7% for D11S449, respectively. CONCLUSION: The high frequency of loss of heterozygosity at 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.

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OBJECTIVE: To analyze the loss of heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC). METHODS: Relatively pure tumor tissues were obtained by microdissetion. PCR was used to perform microsatellite analysis of NPC samples using D11S4946, PYGM, D11S449 and INT-2 as primers, which span the MEN-1 gene. RESULTS: Thirty-six of 38 cases studied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (78.8%). The LOH frequency was 51.5% for INT-2, 45.5% for PYGM and 45.7% for D11S449, respectively. CONCLUSION: The high frequency of loss of heterozygosity at 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.

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Available abstract

OBJECTIVE: To analyze the loss of heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC). METHODS: Relatively pure tumor tissues were obtained by microdissetion. PCR was used to perform microsatellite analysis of NPC samples using D11S4946, PYGM, D11S449 and INT-2 as primers, which span the MEN-1 gene. RESULTS: Thirty-six of 38 cases studied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (78.8%). The LOH frequency was 51.5% for INT-2, 45.5% for PYGM and 45.7% for D11S449, respectively. CONCLUSION: The high frequency of loss of heterozygosity at 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.

Key concepts: Loss of heterozygosity, Nasopharyngeal carcinoma, Biology, Carcinogenesis, Microsatellite, Chromosome, Tumor suppressor gene, Carcinoma

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[Study of loss of heterozygosity at chromosome 11q13 in nasopharyngeal carcinoma]. — Research Paper | ScholarLens