Study of apoptosis in human gastric cancer MGC803 cell and the induction by Paclitaxel

Objective:To investigate the action of insitu apoptosis in occurrence of gastric cancer and the biological effect of Paclitaxel on MGC803 cell line inducing apoptosis.Methods:The apoptosis in the gastric cancer detected with terminal deoxynucl neotidyl transferase mediated dUTP nick end labelling(TUNEL),the MGC803 cell line cultured in medium were treated with Paclitaxel and observed undermicroscope,and their DNA was assayed by gel electrophoresis,Flow cytometry analysis was used to observe the cell cycle,the effect measured telomerase.Results:In situ apoptosis cells in the cancer adjacent tissues inceased;treatment of gastric cancer with Paclitaxel could induce the cells to manifest a typical apoptosis morphology.Their DNA was fractured and a characteristic ladder pattern could be found using electrophoresis,which was associated with arrest of G2/M phase,and inhibited telomerase activity.Conclusion:In vivo the apoptosis of gastric cancer lower,maybe the cells divide quickly and then the cancers occur.In the cancer adjacent tissues,the apoptosis prick up,and in vitro Paclitaxel can induce the apoptosis of MGC803 cells.