Cloning and sequence analysis of melanocortin receptor-4 gene in yak

Melanocortin Receptor-4(MC4R) Gene in Yak was cloned and sequenced,in order to provide theoretical foundation for further research of the corelevantion of MC4R gene in yak and fat traits,location and expression i.e.MC4R gene of yak was amplified using particular primers,cloned by T-A method and sequenced,and spliced by using BioEdit7.0.0.then translated by using DNAMAN5.2.2.All MC4R sequences were aligned and compared in percent identity by using DNAStar6.13.The length of MC4R gene in yak was 1343bp,in which the length of complete coding sequence(CDS) was 999bp,encoding 332 amino acids.Homology of nucleotide sequence between yak and cattle,human,crab-eating macaque,pig,dog,rat,mouse,chicken,zebrafish,goldfish,fugu rubripes and tetra is 99.5%,85.5%,84.9%,88.1%,86.7%,83.9%,83.8%,75.5%,61.2%,59.5%,65.3% and 64.6% respectively.Homology of amino acid sequence of them is 99.1%,91.9%,91.9%,93.1%,92.2%,90.4%,89.8%,84.0%,66.7%,65.7%,67.2% and 66.7% respecxvely.MC4R gene of yak was successfully cloned in this study,indicating it was quite conservative among species.