MUTAGENIC EFFECTS OF ION IMPLANTED RICE SEED

Dry seeds of rice were implanted with 15~30 keV N+ , H+ , Ar+ ion beam of various doses. The biological effects in M1 and mutation in M2 were studied. The results showed that ion beam could induce the variation on the chromosome struc- ture and inhibite mitosis in root tip cell. The chromosomal aberration rate of cells tended to be increased with increase of implanted ion dose. Compared with 60Co 7-rays, ion implantation induced lower rate of cells with chromosome aberration. However, there was a similary inhibitory effect on mitosis between ion beam and 7-rays. The electrophoretic banding patterns of peroxidase enzymes were altered by both mutagens and varied. Frequency of the chlorophyll mutation implanted by ion beam was higher than that induced by 7-rays. Mutation frequencies of heading date and plant height were similar between ion beam implanting and 7-rays irradiation.