SRAP analysis on genetic diversity of Pennisetum
Yao Yun-f
Abstract
Yao Yun-f
Abstract
The genetic relationship of 19 cultivars of Pennisetum were analyzed using SRAP molecule maker.The results showed that the 682 reproducible fragments of SRAP were produced by 38 of 100 pairs of primers;the bands for each pairs of primers to all samples varied from 9 to 22,and 157 fragments(4.13 per pairs of primers)were polymorphism which indicated that there was high genetic diversity among these 19 genotypes.From the results of NTSYS pc 2.01 software cluster analysis,the simple matching coefficient(SM)of the Pennisetumcultivars ranged from 0.53 to 0.82.When the similarity coefficient was 0.60,the cultivars could be grouped into 3 categories,type A,B and C,based on cluster analysis of the SRAP fingerprinting data.When the similarity coefficient was 0.67,type B had two subclasses;type C had three subclasses.Combined with their morphological characteristics,the results could be applied for the objective basis to identify the genetic relationship of Pennisetumresources.
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The genetic relationship of 19 cultivars of Pennisetum were analyzed using SRAP molecule maker.The results showed that the 682 reproducible fragments of SRAP were produced by 38 of 100 pairs of primers;the bands for each pairs of primers to all samples varied from 9 to 22,and 157 fragments(4.13 per pairs of primers)were polymorphism which indicated that there was high genetic diversity among these 19 genotypes.From the results of NTSYS pc 2.01 software cluster analysis,the simple matching coefficient(SM)of the Pennisetumcultivars ranged from 0.53 to 0.82.When the similarity coefficient was 0.60,the cultivars could be grouped into 3 categories,type A,B and C,based on cluster analysis of the SRAP fingerprinting data.When the similarity coefficient was 0.67,type B had two subclasses;type C had three subclasses.Combined with their morphological characteristics,the results could be applied for the objective basis to identify the genetic relationship of Pennisetumresources.
Key concepts: Genetic similarity, Genetic diversity, Biology, Similarity (geometry), Genetic relationship, Pennisetum, Cultivar, Polymorphism (computer science)