2006•Chinese journal of integrated traditional and Western medicineRequires access
Synergetic effect of Yihuoqingyi decoction and recombinant staphlokinase on severe acute pancreatitis in rats
Sha Jian-ping
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Abstract
[Objective] To investigate the effect of recombinant staphlokinase(r-Sak) and the traditional Chinese medicine,Yihuoqingyi decoction(YD),and the synergetic effect of the two kinds of medicines on the therapy of the severe acute pancreatitis(SAP) in rats.[Methods] One hundred and sixty-two male SD rats were divided randomly into the sham-operated group(group A,n=18),the control group(group B,n=36),the YD treatment group(group C,n=36),the r-SAK treatment group(group D,n=36) and the YD plus r-SAK treatment group(group E, n=36).SAP was induced by intraductal administration 5 % sodium taurocholate.Nine rats were chosen randomly from each group for determining the survival rate.The abdomen of nine rats in each group was opened at 6 h,12 h and 18 h after induction of the model for evaluating the pancreatic blood flow and the weight of ascites,and the rats were killed for obtaining the blood and detecting the concentration of serum amylase,lipase.The pancreatic tissues were stained with HE and examined under light microscopy.[Results] The survival count of group A,B,C,D and E rats was 9,2,6,7 and 8 respectively.The concentration of serum amylase,lipase and the weight of ascites of group A,C,D and E significantly decreased,compared with those of group B(P0.05);the concentration of serum amylase,lipase and the weight of ascites of the group reduced,compared with those of the group(P0.05);the concentration of serum amylase,lipase and the weight of ascites of group E decreased,compared with those of group C and D(P0.05).The pancreatic blood flow of group B,C,D and E decreased,compared with that of group A;that of group C,D and E increased,compared with that of group B;that of group D increased,compared with that of group C;that of group C,D and E increased,compared with that of group C,D and E at the three time points.Pathological changes of pancreatic tissue in group C,D and E alleviated in contrast to those of group B.[Conclusion]Both r-Sak and YD have beneficial role for the therapy of rats SAP,and the two kinds of medicines have synergetic effect.