Expression of Transforming Growth factor-β1 and Vascular Endothelial Cell Growth Factor and Their Correlation with Angiogenesi in the Breast Cancer

Objective: To investigate the expression of transforming growth factor-β1(TGF-β1) and vascular endothelial cell growth factor(VEGF) and their correlation with angiogenesi in the breast cancer. Methods: 65 cases of operation excision of breast cancer paraffin blocks and the surrounding normal breast tissue were selected and divided into two groups: group A was the control group, the specimens of which were breast cancer adjacent normal breast tissue; group B was the experimental group, the specimens of which were breast cancer tissue. The expression of TGF-β1 and VEGF were detected by immunohistochemical staining and morphometric study.Micro-vessel density(MVD) were tested by CD34 related antigen as marker of vascular endothelial cell and the correlation of MVD with expression of TGF-β1 and VEGF were analyzed. Results: In 65 cases of breast cancer, the positive expression rate of TGF-β1 was69.23%(45/65), the MVD value of those TGF-β1 positive expression(26.18±4.05) was significantly higher than that in TGF-β1 negative expression(20.13±4.29). The positive expression rate of VEGF was 78.46%(51/65), the MVD value of those VEGF positive expression(25.82±3.61) was also significantly higher than that in the negative group(19.65±6.32)(P0.05). The positive expression rate of VEGF and TGF-β1 showed no statistical difference in different pathological types of breast cancer tissues(P0.05). However, according to the histological grade, lymph node metastasis showed significant correlation, and the higher histological lymph node metastasis was, the higher MVD value was. Conclusion: The expression of TGF-β 1 and VEGF in breast cancer tissue were higher than those in the adjacent normal breast tissue, and were correlated with the angiogenesis in the breast cancer. TGF-β1 and VEGF could be used as the evaluation index of biological behavior such as malignant degree, invasion, metastasis of breast cancer.