The Experiment Study of Nourishing Yin,Invigorating Qi,Extinguishing Wind,Promoting Blood Circulation and Draining Collateral Method of the Sciatic Nerve Schwann Cells Apoptosis of DM Rats

Objective:To observe the effect of nourishing Yin,invigorating Qi,promoting blood circulation,extinguishing wind and draining collateral method of the sciatic nerve Schwann cells apoptosis of DM rats.Methods:60 SD male rats were randomly divided into normal group,model group,western medicine group (methycobal and gliclazide),high dosage,middle dosage and low dosage Jiangtangshuluo Formula group.DM model was mode by STZ abdominal cavity injection.The SINS,HbA1c,sciatic nerve Schwann cells apoptosis,the expression of caspase-3,Bcl-2 and Bax were recorded.Results:The DM rats' sciatic nerve Schwann cells apoptosis,caspase-3,Bax gene protein expressed of the model group rats sciatic nerve higher and the Bcl-2 gene protein expressed lower than the normal group obviously.There were statistics significance between the model group and that all Jiangtangshuluo Formula group and western medicine group to the sciatic nerve Schwann cells apoptosis,caspase-3,Bax and Bcl-2.The effect of high and middle dosage Jiangtangshuluo Formula group to the Schwann cells apoptosis,caspase-3,Bax and Bcl-2 was superior to the western medicine group.The curative effect of high dosage Jiangtangshuluo Formula group was better than the other two Jiangtangshuluo Formula groups.Conclusions:Nourishing Yin,invigorating Qi,extinguishing wind,promoting blood circulation and draining collateral method can improve the levels of insulin and reduce the level of HbA1c of DM rats,inhibit Schwann cells apoptosis.The mechanism maybe that it can inhibit the activeness of caspase-3 and the expression of Bax,and promote the expression of Bcl-2.The effect of the method to each observation target shows the dosage dependence.