Use of Molecular Imprinted Monolithic Column for the HPLC Determination of Melamine in Dairy Products

A molecular imprinted monolithic column was prepared for providing stationary phase for the HPLC analysis of melamine in dairy products,in which the mobile phase was a mixture of 10% aqueous methanol solution and 0.1% aqueous acetic acid solution(5:95,V/V) at a flow rate of 0.5 mL/min and the detection wavelength was set at 240 nm.There was a good linear relationship between HPLC peak area and melamine concentration over the range from 1.0 to 100.0 μg/mL(r = 0.9987).Average recoveries for melamine in a blank sample spiked at 3 levels were between 87.0% and 100.5%,with a RSD of less than 5%(n = 6).In conclusion,this method is easily operated,highly selective and has no requirement of complicated sample pretreatment and is applicable for the determination of melamine residue in dairy products.