The Effect of Octreotide on Proliferation and P-glycoprotein Expression of SGC-7901 Cell Line

Objective:To evaluate the effect of different concentrations of octreotide alone or combined with fluorouracil (5-Fu) on the growth of human gastric cancer line SGC-7901, observe whether or not the cell proliferation cycle is arrested .and apoptosis induced, and explore the effect of octreoride on expression of P-glycoprotein(P-gp). Methods: In vitro, octreoride alone or combined with 5-Fu was added in SGC-7901. MTT assay was performed to observe the degree of growth inhibition of cells and flow cytometry was applied to detect cell proliferation cycle, apoptosis and expression of P-gp. Results :The growth of cell SGC-7901 was inhibited by octreoride in vitro.The inhibition depended on dose and time and possessed the character of saturation.Neither apoptosis nor cell cycle arrest was induced by different concentrations of octreotide. Antagonism was observed when octreotide and 5-Fu were combined. The expression of P-gp in SGC-7901 could significantly be downregulated by octreotide. Conclutions: Octreotide can inhibit proliferation of tumor cells neither by apoptosis nor cell arrest. Considering that octreotide combined with 5-Fu may have antagonism, combining application was not recommended. The expression of P-gp in SGC-7901 is downregulated by octreotide, which may reverse drug-resistance of tumor cells.