2006•Journal of Xinjiang Agricultural UniversityRequires access
Expression of Chicken Mature IL-18 Gene in E.coli and Preparation of Its Antiserum
Hailing Zhang, Ran Duo-liang
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Abstract
Chicken IL-18 mature protein gene was amplified from IL-18 recombinant plasmid by PCR,and was subcloned into prokaryote expression vector pPROEX~(TM)HTa and then was transformed into E.coli DH5α and was induced with IPTG at 37 ℃,SDS-PAGE analysis showed induced products about 26 ku.The recombinant mChIL-18 was expressed in form of inclusion body with the yield accounting for(21.95%) of total bacterial proteins.The matural protein of Chicken IL-18 was purified by Ni-NTA resin.The antiserum was obtained by immunizing SPF chicken with the purified recombinant protein.Western blot result showed the antiserum raised against the recombinant mChIL-18 in chicken could react to protein expressed specifically.ELISA detection showed the antigenicity of the fusion protein was satisfactory.