Study on Salt-tolerant Features of A Moderately Halophilic Bacterium Halomonas sp. STSY-3

[Objective] The study aimed to provide the method and parameter guidance for the screening of the engineering halophilic bacteria and also provide the necessary theoretical basis for the selecting,problem diagnosis and process optimization of the engineering bacterium during the biochemical treatment process of high salt wastewater through the study on salt-tolerant features of a moderately halophilic bacterium Halomonas sp.STSY-3.[Method] With the moderately halophilic bacterium Halomonas sp.STSY-3 as the tested material,the effects of salinity,inoculation amount,anions and cations,osmotic pressure impact on the growth feature of this bacteria were determined by using the spectrophotometry.[Result] Halomonas sp.STSY-3 had a strong adaptability to the changes of salinity,the salinity range for the growth of this bacteria was 1%-11%(NaCl,W/V) and the optimum salinity for the growth was 7%(NaCl,W/V).Under a series of NaCl concn.,the increasing of inoculation amount could effectively enhance the growth of this bacteria at different salinity.Among 12 kinds of anions and cations,only Cl-and Na+ were the optimum ions for its growth and the bacteria also had stronger tolerance to the SO42-.[Conclusion] The study provided the basic data of the biochemical treatment of high-salt wastewater and the determination method for the salt-tolerant features.