Isolation and Identification of a Moderately Halophilic Bacterium and Its Bioaugmention in Intensifying High-salty Organic Wastewater Treatment

In order to study the application of moderately halophilic bacterium in the hypersaline organic wastewater treatment,a moderately halophilic bacterium YS-1 was isolated from the salty water of a Ludaokou salina of Weihai in China.The polyphasic taxonomy study was conducted by identifying its morphological features by AFM by studying the features of culture,measuring the physiologic-biochemical index and analyzing 16 S rDNA sequence homology.Phylogenetic analysis based on 16 S rDNA gene sequences showed that the closest relative of the strain was Halomonas sp.(AB167061).Experiments of intensifying hypersaline organic wastewater treatmented processing in the SBR demonstrate a significant result. The wastewater salt content of initial wastewater was 12% and the initial COD cr was 1 494 mg/L.After 72h's,the removal efficiency of COD cr achieved 90.0%,and after 120 h,it achieved 98.1%.The experiments also showed that the hypersaline organic wastewater treatment had the feasibility acclimating of halophilic bacteria and running of processing system.