On the biological features of the disease producing germ of Fusarium moniliforme var.subglutinans

【Objective】 The study provided some instructions to prevent and cure corn top-rotten disease.【Method】 A research on the effect of temperature,pH,illumination,carbon and nitrogen sources on mycelial growth,sporulation and spore germination of Fusarium moniliforme var.subglutinans.was conducted.【Result】 The optimum temperature range of mycelial growth,spore germination,and producing spore germination were 20-25 ℃,25-30 ℃,25 ℃ respectively.The mycelial could grow and produce spore germination on pH 4-13,the optimum pH for hypha growth was pH 5-12,the optimum acid range for spore germination pH 7-9,and the optimum acid range for producing spore germination pH 6-8.The mycelial could grow on the provided 10 carbons,and grew fast in the culture medium with carbons form solubleamy and mannite.There big differences from other 8 carbon sources.The effects of the 10 carbons on the spore germination were almost the same;the spore geminated best in the culture medium whose carbon came from Glycerin,and slowest in the culture medium whose carbon came from Lseminose.The mycelial could grow and germinate in the 8 nitrogen sources.The hypha grew fast in the culture medium whose nitrogen sources came from Ammonium nitrate,and produce the most sporulation,which is quite different from other 7 nitrogen sources;The mycelial germinated the most in the culture medium whose nitrogen sources came from Glycin,which was also different from other 7 ones;continuous illumination,darkness and alternative of the two didn't have a significant differences on the growth of the mycelial;12 h alternatives of illumination made a striking difference on the spore germination;continuous illumination produced 100% spore germination,while darkness resulted in only 10.8%,the difference quite striking.【Conclusion】 The optimum temperatures range of mycelial growth,spore germination,and producing spore germination were 20-25 ℃,25-30 ℃,25 ℃ respectively;The optimum pH range were 5-12,7-9,6-8.The mycelial could grow on the provided 10 carbons,and grew fast in the culture medium with carbons form solubleamy and mannite.