Study of The Level of TNF-α,LIF and HGF Secreted by Bone Marrow Stromal Cells in Psoriatic Patients

Objective To reveal the abnormity of bone marrow microenvironment of psoriasis patients by comparing the level of TNF-α、LIF and HGF secreted by bone marrow stromal cells in patients and control.Methods Separate bone marrow mononuclear cells of patients and control by density gradient centrifugating.Bone marrow stromal cells were cultured with the method of adherent.When the cells were cultured for three passages and 72h,bone marrow stromal cells and supernatant liquid were collected.The phenotypes of cells were identified by flow cytometry and the level of TNF-α,LIF and HGF were detected by ELISA kits.Results The purity of bone marrow stromal cells was over 90%.The level of TNF-α,LIF and HGF in patients was significantly lower than that in control(P0.05).Conclusions Some of the cytokines which secreted by psoriatic bone marrow stromal cells are abnormal.This shows that psoriatic bone marrow microenvironment may be abnormal.