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Study on Antioxidant Activity of Whey Protein Hydrolysates

Xiong You-ling

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Abstract

Free radical-scavenging ability and antioxidant activity of whey protein hydrolysates were examined in different systems,including reducing ability,inhibition of PV(peroxide value) and TBARS(thiobarbituric acid-reactive substances),metal ion chelating activity and DPPH radical-scavenging activity.The results showed that the hydrolysates from 5% whey protein hydrolyzed by alkaline protease(Alcalase) at the amount of 2% for 5h have the strongest antioxidant activity,which is influenced by substrate concentration,hydrolysis time and protein solubility.

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Free radical-scavenging ability and antioxidant activity of whey protein hydrolysates were examined in different systems,including reducing ability,inhibition of PV(peroxide value) and TBARS(thiobarbituric acid-reactive substances),metal ion chelating activity and DPPH radical-scavenging activity.The results showed that the hydrolysates from 5% whey protein hydrolyzed by alkaline protease(Alcalase) at the amount of 2% for 5h have the strongest antioxidant activity,which is influenced by substrate concentration,hydrolysis time and protein solubility.

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Available abstract

Free radical-scavenging ability and antioxidant activity of whey protein hydrolysates were examined in different systems,including reducing ability,inhibition of PV(peroxide value) and TBARS(thiobarbituric acid-reactive substances),metal ion chelating activity and DPPH radical-scavenging activity.The results showed that the hydrolysates from 5% whey protein hydrolyzed by alkaline protease(Alcalase) at the amount of 2% for 5h have the strongest antioxidant activity,which is influenced by substrate concentration,hydrolysis time and protein solubility.

Key concepts: Chemistry, TBARS, Hydrolysate, Antioxidant, Hydrolysis, Thiobarbituric acid, Whey protein, DPPH

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