CONSTRUCTION OF RECOMBINANT PROKARYOTIC PLASMID AND EXPRESSION OF SCHISTOSOMA JAPONICUM NOVEL GENE IN ESCHERICHIA COLI

Objective To identify the Schistosoma japonicum novel gene JAYL0230. Methods JAYL0230 cDNA was subcloned into prokaryotic plasmid pET28a(+). Then the recombinant fusion protein was induced by IPTG in bacterial culture. SDS PAGE analysis and Western Blot were processed to identify the character of the recombinant protein. Results The recombinant plasmid was constructed successfully. SDS PAGE revealed the fusion protein's molecular mass was consistent with the prediction by biological software. Western Blot analysis indicate that the recombinant protein is immune reactive. Conclusion The protein encoded by JAYL0230 gene is immune reactive, which suggests the novel gene of S. japonicum is worthy of further study.