Identification and expression of signaling protein 14-3-3 of Schistosoma japonicum(Chinese mainland strain) in prokaryotic cells

Aim To construct a recombinant plasmid pET28a-Sj14-3-3,and detect the immunoactivity of the expression in E coli Methods Subclone the Sj14-3-3 encoding gene into the downstream of the T7 promoter of vector pET28a,transform the recombinant plasmid into competent cells of E coli DH5α and BL21,and induced by IPTG The cell lysates were analysed with SDS-PAGE followed by Western blot Results The recombinant expression plasmid pET28a-Sj14-3-3 was successfully constructed SDS-PAGE and Western blot showed a fusion protein with the molecular weight of 32 4KDa and the expressed product had the same immunoactivity as native 14-3-3 protein Conclusion Signaling 14-3-3 protein of Schistosoma japonicum(Chinese mainland strain) was high expressed in prokaryotic cell and identified by anti-14-3-3e immunogen The primary work provided a new approach to molecular candidate vaccine and signal transduction of Schistosome