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EFFECTS OF CHIGUO OIL STEROL ON PROLIFERATION AND DIFFERENTIATION OF RAT PREADIPOCYTES
Shi Jie-ping
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Abstract
To investigate effects of Chiguo oil sterol on proliferation and differentiation of rat adipocytes and to elucidate its potential mechanism,rat's primary preadipocytes invitro were cultured.Treated preadipocytes with 0μmol/L(control group),2 μmol/L,4 μmol/L,8 μmol/L Chiguo oil sterol.Cell proliferation was analyzed by MTT assay.The degree of adipogenesis and differentiation were measured by Oil Red O staining extraction assay and the expression of peroxisome proliferation activated receptor-γ2(PPARγ2)mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).It was demonstrated that the optical density(OD)of MTT were decreased,especially treated by 4 μmol/L and 8 μmol/L.The OD of Oil Red O staining and the expression of PPARγ2 mRNA were all decreased after treated by 4 μmol/L and 8 μmol/L Chiguo oil sterol(P0.05).