Adenoviral vector-mediated transfer of NT-3 genes into rat Schwann cells in vitro

Objective To investigate expression of neurotrophin 3(NT 3)gene in cultured Schwann cells introduced by adenoviral vector in vitro.Methods The recombinant adenoviral vector for NT 3(Ad NT 3)was propagated in 293 packaging cells. Viral titers were determined by tissue culture infectious dose 50 (TCID 50 ) methods . The primary culture and purification of Schwann cells was established and the efficiency of transfection of Ad NT 3 was determined by reverse transcriptase polymerase chain reaction(RT PCR).Results High titers of recombinant adenoviral particles after propagation of Ad NT 3 in 293 cells were obtained. NT 3 mRNA transcription could be detected by RT PCR in SCs 24 hours following infection with Ad NT 3.Conclusion It is demonstrated that Ad NT 3 transfected SCs can express NT 3 mRNA.