2004•Journal of Sun Yat-sen UniversityRequires access

Transfection of Adenovirus-mediated Neurotrophin-3 Gene into Schwann Cells

Jia Guo, Sun Yat

Abstract

To construct neurotrophin 3(NT 3) genetically modified Schwann cells(SCs). The NT 3 gene recombinant adenovirus(AdvNT 3) were amplified, purified,and determined. Then, it was used to genetically modify SCs. The NT 3 expression of the NT 3 genetically modified SCs(NT 3 SCs) was determined immunocytochemically, and the amount of NT 3 in the cultural media of NT 3 SCs was determined with ELISA method.The exogenous gene sequence of the obtained AdvNT 3 was equal to that of the NT 3 gene in rat. Compared to the normal SCs, the positive expression of NT 3 in NT 3 SCs was enhanced, and the amount of NT 3 in the cultural media of NT 3 SCs was increased. [Conclusion]Mediated by adenovirus, NT 3 overexpressed genetically modify Schwann cells can be obtained.

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Key concepts: Transfection, Neurotrophin-3, Molecular biology, Recombinant DNA, Biology, Gene, Neurotrophin, Genetically modified organism