Amplification of Sj314C10 gene and construction of DNA vaccine of Schistosoma japonicum

The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290). The bioinformatics sequencing revealed that the gene was a novel gene of S.japonicum, and it was named as Sj314C10(S.japonicum 314C10 cloning).The codons protein region of the gene was cloned into pcDNA3-vector and recombinant plasmid was transformed into E.coli DH5α. Positive clone of recombinant plasmid bacterial E.coli DH5α was selected to identify. The result (agreed) with what was anticipated.Thus a recombinant expression plasmid was constructed successfully. It was made foundation for further studying the gene’s function.