Comparison on Methods for Pathogenicity Test of Seed-Borne Fungi in Soybean

Seed-borne fungi could decrease germination,activity,quality and commercial grade by infecting soybean seed.For discussing the effect of different inoculation methods on pathogenicity of seed-borne fungi,seed inoculation method and plant inoculation methods( apical cut inoculation method,wound inoculation method,stem cut inoculation method and hypocotyl inoculation method) were compared at laboratory condition. The result showed that seed-borne fungi could infect soybean under each inoculation method. The differences of pathogenicity between seed-borne fungi were significantly distinguished by all inoculation methods. Compared with other three plant inoculation methods,apical cut inoculation method was simple,effective and accurate. Seed inoculation method was fit for room test and the test cycle was short. It was also more convenient and efficient than plant inoculation.