Effect of Ginsenosides on Ischemia Reperfusion Injury of Skeletal Muscle And Morphologic Study

Objective:To study the protective effect and mechanism of Ginsenosides on ischemia reperfusion injury of skeletal muscle.Methods:The experimental model of ischemia reperfusion injury in extremity were produced in 20 healthy adult rabbits which were randomly divided into experimental and control groups(each of 10 rabbits).Normal saline injection and Ginsenosides were infused intravenously into rabbits of control group,experimental group respectively just before blood reperfusion.At preischemia,2 hours post-ischemia,1 hour post-reperfusion and 3 hours post-reperfusion,sample of blood was collected from the femoral veins respectively for measurements of lactic dehydrogenase(LDH),creatine kinase(CK) by means of biochemical methods in every rabbit.Meanwhile the tibialis anterior muscles were harvested for the measurements of Wet/Dry ratio of skeletal muscle and for the microscope and ultrastructure examinations by using optical microscope and electronic microscope respectively.Results:After reperfusion,the contents of LDH,CK were dramatically higher in control group(P0.01) but were not in experimental group(P0.05).The Wet/Dry ratio of skeletal muscle had not obvious difference in every group(P0.05) before reperfusioin.The skeletal muscle injury of experimental groups,after reperfusion was of a mild degree than of control group in light microscope and electronic microscope examinations.Conclusion:Ginsenosides can effectively improve soft tissue swelling and protec skeletal muscle from ischemia reperfusion injury.