2012•Zhongguo youzhiRequires access

Pretreatment method for detecting free gossypol content in cottonseed meal by HPLC

Weinong Zhang

Abstract

Pretreatment method for detecting free gossypol content in cottonseed meal by high performance liquid chromatography(HPLC) was studied.Free gossypol was extracted from cottonseed meal assisted with ultrasonic using 70% acetone as solvent.The effects of extraction conditions on determination of free gossypol content were systematically investigated by HPLC.The optimal pretreatment conditions were as follows: ratio of solvent to solid 26 ∶ 1,ultrasonic time 60 min,extraction once.Under the optimal pretreatment conditions,the determination result had virtues of good stability(RSD=2.06%) and repeatability(RSD=1.62%),and the recovery rate was in the range of 97.89%-101.17%.This method could be used for determination of free gossypol content in cottonseed meal.

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Key concepts: Gossypol, Chromatography, Cottonseed meal, High-performance liquid chromatography, Repeatability, Cottonseed, Acetone, Chemistry