Relationship between hepatic pathological changes and levels of serum HBV-DNA,hepatic fibrosis markers in chronic hepatitis B patients

Aim To discuss the relationship between hepatic pathological changes and the quantitative determination of HBV-DNA and the relationship between liver fibrosis and serum levels of hepatic fibrosis markers in patients with chronic hepatitis B.Methods 59 patients were divided into two groups according to the serum immunological markers,HBeAg-positive group and HBeAg-negative group.These cases had liver histopathologic assay,serum HBV-DNA by fluorescent quantitative PCR and serum levels of hepatic fibrosis markers by RIA.Results There was significant difference in the serum level of HBV-DNA among various inflammation grades in HBeAg-positive group.No significant difference was found between the serum levels of HBV-DNA in the different stages of liver cirrhosis.These levels in HBeAg-negative group have no statistics discrepancy.The increase in serum HA,PCⅢ,CIV was correlated with the progression of liver fibrosis.Conclusions The serum level of HBV-DNA in HBeAg-positive group gradually increased with the development of inflammation.Because no significant correlations were shown between HBV-DNA quantitation and hepatic pathological change in HBeAg-negative group,liver histology is vatuable in diagnosis of liver damage with different HBV-DNA.Combined examing HA,LN,PCⅢ,CIV has diagnostic value of hepatic fibrosis.