Clinical application of serum markers of liver fibrosis and copy of HBV-DNA in the diagnosis of hepatic fibrosis

Objective To study the clinical significance of the serum of liver fibrosis in the liver fibrosis nontraumatic check,and the relationship between changer of blood HBV DNA viral load and serum liver fibrosis markers levels in patients with chronic hepatitis B.Methods Serum levels of HA,LN,CⅣ,PCⅢ in 143cases of chronic hepatitis B were detected by radioimmunoassay(RIA),HBV-DNA were measured by the way of nucleic acid amplification fluorescent quantitation(FQ-PCR),and hepatitis b virus markers(HBVM) were tested by using ELISA.Results There was significant difference in quantity of serum HA and PCⅢ of liver fibrosis four index of liver fibrosis in mild or moderate or severe chronic hepatitis and cirrhosis in turn increase trend(P0.05).In the hepatitis b virus markers,serum markers of liver fibrosis of HBeAg positive and HBV-DNA positive group was minimum,serum markers of liver fibrosis of HBeAg negative and HBV-DNA negative group was medium on the high side,and serum markers of liver fibrosis of HBeAg negative and HBV-DNA positive group was the highest.Conclusion HBeAg negative chronic hepatitis b patients with HBV-DNA positive and age,liver disease continued to develop,and fibrosis was increasing.The liver fibrosis four index and HBV-DNA united detection have an important value on clinical judgment of liver fibrosis degree and guiding anti fibrosis and antiviral treatment.