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2004•Acta Agriculturae Boreali-SinicaRequires access

Construction of AFLP Analysis System in Cucumber

Guihua Zhang

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Abstract

AFLP (Amplified Fragment Length Polymorphism) analysis system was established with 24 cucumber self - bred lines. Several factors, include the quantity of genome DNA for cutting, the diluted times of pre - amplifications and the number of selective nucleotide in primer, were optimized. With the optimized system, the AFLP fingerprinting of 24 cucumber materials amplified by primer combination P12M12 have been obtained, which had 35 clear bands, and 4 of them were polymorphic.

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What this paper is about

AFLP (Amplified Fragment Length Polymorphism) analysis system was established with 24 cucumber self - bred lines. Several factors, include the quantity of genome DNA for cutting, the diluted times of pre - amplifications and the number of selective nucleotide in primer, were optimized. With the optimized system, the AFLP fingerprinting of 24 cucumber materials amplified by primer combination P12M12 have been obtained, which had 35 clear bands, and 4 of them were polymorphic.

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Available abstract

AFLP (Amplified Fragment Length Polymorphism) analysis system was established with 24 cucumber self - bred lines. Several factors, include the quantity of genome DNA for cutting, the diluted times of pre - amplifications and the number of selective nucleotide in primer, were optimized. With the optimized system, the AFLP fingerprinting of 24 cucumber materials amplified by primer combination P12M12 have been obtained, which had 35 clear bands, and 4 of them were polymorphic.

Key concepts: Amplified fragment length polymorphism, Primer (cosmetics), Biology, Genetics, Genome, DNA profiling, DNA, Gene

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