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Establishment and optimization of AFLP analysis system in rabbit

Dong JinHua, Fan XinZhong, Dong Hui, Xibo Qiao, Sun ZongGuo, Wu Hongchao, Kaiyong Xu

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Abstract

AFLP(Amplified Fragment Length Polymorphism) analysis system was established with rabbit.The results of several reactions during AFLP analysis were detected such as DNA extraction,double enzymes digestion,adapter ligation,preamplification,selective amplification and silver staining.We Obtained 15 combinations of primers,which had clear and strong amplification bands with bright background.The factors which affected AFLP analysis were discussed.

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What this paper is about

AFLP(Amplified Fragment Length Polymorphism) analysis system was established with rabbit.The results of several reactions during AFLP analysis were detected such as DNA extraction,double enzymes digestion,adapter ligation,preamplification,selective amplification and silver staining.We Obtained 15 combinations of primers,which had clear and strong amplification bands with bright background.The factors which affected AFLP analysis were discussed.

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Available abstract

AFLP(Amplified Fragment Length Polymorphism) analysis system was established with rabbit.The results of several reactions during AFLP analysis were detected such as DNA extraction,double enzymes digestion,adapter ligation,preamplification,selective amplification and silver staining.We Obtained 15 combinations of primers,which had clear and strong amplification bands with bright background.The factors which affected AFLP analysis were discussed.

Key concepts: Amplified fragment length polymorphism, Adapter (computing), Biology, DNA, Molecular biology, DNA extraction, genomic DNA, Polymerase chain reaction

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