Expressive changes of NF-к Bp65 and ICAM-1 in lung ischemia/reperfusion injury in rabbits

Objective: To explore changes and significance of NF-кBp65 and ICAM-1 during lung ischemia reperfusion injury in rabbits. Methods: Rabbit lung model of ischemia reperfusion injury was constituted in vivo. Fifty rabbits were randomly divided into five groups: control group, ischemia group 1 h and ischemia reperfusion group 0.5 h,1 h and 3 h. NF-кBp65 expression was measured with immunohistochemistry, ICAM-1mRNA expression was detected with in situ hybridization histochemistry and leukocyte infiltration was detected with MPO activity. Results: NF-кBp65 expression after 0.5 h of reperfusion was obviously higher than that in group C (P0.01); After 1 h of reperfusion, MPO activity and ICAM-1mRNA expression were significantly higher than that in group C (P0.01) and they increased gradually during reperfusion. Significant positive correlation was observed among NF-кBp65 activation, ICAM-1 mRNA expression and MPO activity, respectively(P0.01). Conclusion: NF-кBp65 can regulate the expression ICAM-1mRNA and advance sequestration of PMN during lung ischemia reperfusion injury.