Experimental Study of FK_(506) on the Regeneration of Nerve Allografts in Rabbits

Objective To study the effects of FK_(506) on the regeneration of nerve allografts after cryomanagement in rabbits.Methods Thirty-six heath adult New Zealand white rabbits were used in this study. Four rabbits were selected as donors. Bilateral sciatic nerves were taken out from the rabbits and cryopreserved in a-70℃ refrigerator for 2 weeks. The other of thirty-two rabbits as recipients were randomly divided into two groups (experiment and control). 10mm defects was created in left sciatic nerve , the experiment and control of the nerve allograft for bridging the defect of peripheral nerves was done after rapid thawing. Then management as follow: The experiment group: local injecting FK_(506) (10ug/d×14d).The control group: not any management. Regeneration of the sciatic nerve for all animals were examined by histology、ultrastructure、 morpholog 、immunology and electrophysiologic study method.Results The acute reject reaction was not found. The statistical difference of nerve conduction velocity(NCV) and image pattern analysis in the two groups was significant in 12 weeks after operation(P0.05). The regeneration were evident in two groups under the observation of optical and electronic microscrope. Nerve regeneration in the experiment group was superior to that in the control group.Conclusion The results reveal that FK_(506) stimulated the regeneration of allograft nerves after cryomanagement.