Experimental study of ganglioside on the regeneration of nerve allografts in rabbits

Objective To study the effects of ganglioside (GM1) on the regeneration of peripheral nerve after allografts in rabbits.Methods Thirty-six heath adult New Zealand white rabbits were used in this study. Four rabbits were selected as donors. Bilateral sciatic nerves were taken out from them and cryopreserved in a -86℃ refrigerator for 2 weeks. The other thirty-two rabbits as recipients were divided into two groups (experiment and control) randomly. 10mm defect was created in left sciatic nerve , the experiment and control of the nerve allograft for bridging the defect of peripheral nerves were done after rapid thawing.The rest managements were as follow:(1)The experiment: local injecting GM 1 (1mg/d×14).The control:not any management;(2)Regeneration of the sciatic nerve in both groups were examined electrophysiologically, hislologically, and immunologically.Results The acute reject reaction was not found.analysis between the two groups was significant in 12 weeks after operation(P0.05). Nerve regeneration in the experiment group was superior to that in the control group.Conclusion The results reveal that ganglioside stimulated the regeneration of allograft peripheral nerves.