Effect of multiple single-nucleotide variants in VKORC1 gene on the maintenance doses of warfarin

Objective To investigate the effect of vitamin K epoxide reductase complex subunit 1(VKORC1) gene polymorphism with multiple single-nucleotide variants on the maintenance doses of warfarin in Chinese Han population.Methods VKORC1-1639 G/A,1173C/T and 497T/G polymorphisms in 125 warfarin-treated patients were detected by PCR-RFLP technology.The other data were collected by clinical follow-up.Results The frequency of VKORC1-1639 AA(87.2%),AG(12%) and GG(0.8%) genotype in the patient was the same as that of 1173 TT,TC and CC-type.VKORC1-1639A/G(1173T/C) variants with AA (TT) genotype required less warfarin maintenance dose than AG (TC) or GG (CC) genotype[(2.65±0.90) mg/d vs.(4.88±2.12) mg/d](P0.01).497T/G polymorphism had little impact on warfarin maintenance dose.Conclusion VKORC1-1639 or 1173 variants is closely related with the warfarin maintenance doses.The majority of Chinese Han population were AA or TT genotype,wich may explain why Chinese Han people require less warfarin dose.