Studies on an Enzymatic Hydrolysis Technique of Soybean Protein

Hydrolysis of soybean with acid protease As 3.350 was studied, conditions of pretreatment of raw materials and enzymatic hydrolysis technique were determined.The results of the test showed that the optimum conditions of pretreatment of soybean was 5% HCl and soybean powder at the ratio of 1∶4, treated under 90℃ for 3.5 hour. The optimum condition of enzymatic hydrolysis was the enzyme concentration 6000u per gram of substrate, treated under 45℃ with pH3.0 for 9h. In addition,the soybean powder passed through a 70 mesh sieve before pretreatment and CaCl 2 2×10 -3 mol/L was added to process enzymatic hydrolysis and stirred, then the productivity of amino acid nitrogen could reach 70% and the amino acid nitrogen increased to 0.9g/100mL.