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Preparation of Soybean Protein Hydrolysate for Soybean Protein Adhesive

Zhu Ji

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Abstract

The 20% soybean meal solution was selected to study the hydrolysis properties under the action of pepsin. The hydrolysis properties of the soybean meal solution were considered with the enzymatic hydrolysis temperature,hydrolysis time,pH and the adding amount of enzymes. The effect of heat treatment before enzymatic hydrolysis was also studied. Heat treatment improved hydrolysis properties. After preheated at 90℃ for 10 minutes,and then hydrolyzed at pH1. 8,37℃ for 3 h with the enzyme adding amount of 14 000 U·g- 1,the hydrolysis degree of soybean solution reached 23. 4%,which could be used as the soybean protein hydrolysate for preparing the soybean protein adhesives.

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What this paper is about

The 20% soybean meal solution was selected to study the hydrolysis properties under the action of pepsin. The hydrolysis properties of the soybean meal solution were considered with the enzymatic hydrolysis temperature,hydrolysis time,pH and the adding amount of enzymes. The effect of heat treatment before enzymatic hydrolysis was also studied. Heat treatment improved hydrolysis properties. After preheated at 90℃ for 10 minutes,and then hydrolyzed at pH1. 8,37℃ for 3 h with the enzyme adding amount of 14 000 U·g- 1,the hydrolysis degree of soybean solution reached 23. 4%,which could be used as the soybean protein hydrolysate for preparing the soybean protein adhesives.

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Available abstract

The 20% soybean meal solution was selected to study the hydrolysis properties under the action of pepsin. The hydrolysis properties of the soybean meal solution were considered with the enzymatic hydrolysis temperature,hydrolysis time,pH and the adding amount of enzymes. The effect of heat treatment before enzymatic hydrolysis was also studied. Heat treatment improved hydrolysis properties. After preheated at 90℃ for 10 minutes,and then hydrolyzed at pH1. 8,37℃ for 3 h with the enzyme adding amount of 14 000 U·g- 1,the hydrolysis degree of soybean solution reached 23. 4%,which could be used as the soybean protein hydrolysate for preparing the soybean protein adhesives.

Key concepts: Hydrolysate, Hydrolysis, Enzymatic hydrolysis, Chemistry, Soybean meal, Pepsin, Enzyme, Chromatography

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