Antioxidation Activity and Contents of Total Flavonoids from Different Tissues of Celery

In this paper,the total flavonoids contents of 70 % ethanol extracts from three different tissues of celery were determined by the Al(NO3)3 complexation spectrophotometric method.Using VC as a positive control,antioxidant activity of the extracts were tested and compared by hydroxyl radicals(.OH) scavenging activity,superoxide anion radical(O2-.) scavenging activity,nitrite radical(NO2-) scavenging activity and reducing power.Results indicated that the total flavonoids contents in different tissues of celery had significant difference,the total flavonoids content in leaves was highest(40.71 mg/g),followed by that in stems(10.82 mg/g),and the third was in roots(7.53 mg/g).All the three extracts exhibited strong antioxidant activity in a concentration-dependent fashion in the various antioxidant system.The extracts from celery leaves had the strongest ability to scavenge.OH and O2-.free radicals and reducing power,and the extracts from celery stems had the strongest ability to scavenge NO2-,all the three extracts from celery leaves,stems and roots presented higher.OH scavenging activity than VC.