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TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA

Lu Yi

Open publisher page 1 citations

Abstract

Objective To establish a real time PCR assay based on Taq Man chemistry for detection of measles virus RNA. Methods The primers and probes were screened, and reactive condition was optimized. Results The specificity for detection of measles virus RNA was high, and the sensitivity was 0 1 TCID 50 . It only took three hours to complete the whole course, while the chance contamination decreased obviously compared to conventional PCR. Some clinic samples were detected by this method, and the results were satisfactory. Conclusions The TaqMen based real time PCR is rapid,sensitive and reliable for detection of measles virus.

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What this paper is about

Objective To establish a real time PCR assay based on Taq Man chemistry for detection of measles virus RNA. Methods The primers and probes were screened, and reactive condition was optimized. Results The specificity for detection of measles virus RNA was high, and the sensitivity was 0 1 TCID 50 . It only took three hours to complete the whole course, while the chance contamination decreased obviously compared to conventional PCR. Some clinic samples were detected by this method, and the results were satisfactory. Conclusions The TaqMen based real time PCR is rapid,sensitive and reliable for detection of measles virus.

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Available abstract

Objective To establish a real time PCR assay based on Taq Man chemistry for detection of measles virus RNA. Methods The primers and probes were screened, and reactive condition was optimized. Results The specificity for detection of measles virus RNA was high, and the sensitivity was 0 1 TCID 50 . It only took three hours to complete the whole course, while the chance contamination decreased obviously compared to conventional PCR. Some clinic samples were detected by this method, and the results were satisfactory. Conclusions The TaqMen based real time PCR is rapid,sensitive and reliable for detection of measles virus.

Key concepts: Measles virus, TaqMan, Real-time polymerase chain reaction, Virology, Measles, Virus, RNA, Biology

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