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TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA

Yan Feng

Open publisher page 1 citations

Abstract

Objective To establish a TaqMan-based real-time PCR assay for detection of measles virus RNA,the specific primers and probes were selected and the reactive condition was optimized. Methods The specificity of the assay was high and the sensitivity of the assay was 0.1TCID_(50).The course of extraction of the measles virus RNA and the real-time PCR only took three hours. Results It had the advantages of contamination control,automation,and direct virus quantification.The applicability of this method was evaluated with measles virus and clinical samples and the results was satisfied. (Conclusion )This TaqMan-based real-time PCR was a reliable,sensitive,specific tool for molecular diagnosis of measles virus.

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What this paper is about

Objective To establish a TaqMan-based real-time PCR assay for detection of measles virus RNA,the specific primers and probes were selected and the reactive condition was optimized. Methods The specificity of the assay was high and the sensitivity of the assay was 0.1TCID_(50).The course of extraction of the measles virus RNA and the real-time PCR only took three hours. Results It had the advantages of contamination control,automation,and direct virus quantification.The applicability of this method was evaluated with measles virus and clinical samples and the results was satisfied. (Conclusion )This TaqMan-based real-time PCR was a reliable,sensitive,specific tool for molecular diagnosis of measles virus.

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Available abstract

Objective To establish a TaqMan-based real-time PCR assay for detection of measles virus RNA,the specific primers and probes were selected and the reactive condition was optimized. Methods The specificity of the assay was high and the sensitivity of the assay was 0.1TCID_(50).The course of extraction of the measles virus RNA and the real-time PCR only took three hours. Results It had the advantages of contamination control,automation,and direct virus quantification.The applicability of this method was evaluated with measles virus and clinical samples and the results was satisfied. (Conclusion )This TaqMan-based real-time PCR was a reliable,sensitive,specific tool for molecular diagnosis of measles virus.

Key concepts: TaqMan, Measles virus, Real-time polymerase chain reaction, Virology, Measles, Virus, RNA extraction, Biology

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