Inhibition of Listeria monocytogenes growth by using combined bacteriocin-producing strains in raw beef

The synergy inhibition of two lactic acid bacteria strains,Lactobacillus curvatus and Pediococcus acidilactici H,on the growth of Listeria monocytogenes in frozen beef was investigated.A Lactobacillus curvatus strain(producing the bacteriocin sakacin P)and a Pediococcus acidilactici H(producing bacteriocin pediocin AcH)were inoculated alone or together in a Listeria-seeded raw pork meat which was stored at 4℃ for 6 weeks.The growth of Listeria monocytogenes was controlled by adding either LAB strain alone,yet the rebound appeared after one-week storage.However,the time of inhibition was prolonged by adding combined these two LAB strains to 6 weeks,after when the Listeria monocytogenes had a rebound with the cfu count lower than 10cfu·g-1.Therefore,using the combined these two LAB strains can effectively delay the rebound of Listeria monocytogenes in the beef.