Effect of VEGF MCAb in ischemia-reperfusion injury in pancreas of rats

Objective: To study the effect and mechanism of VEGF MCAb in preventing ischemia-reperfusion injury.Methods: Ischemia-reperfusion models were made by ligated both the anterior menseneric artery and the celiac artery of 70 rats.The expression of TNF-α,VEGF was detected by ELISA.Apoptosis of pancreatic acinar cells was examined by terminal deoxynucleotidyl transferase mediated dUTPbiot in nick end labeling(TUNEL) method.The expression of Fas,FasL mRNA and protein were detected by Real-Time PCR,Western Blot and immunohistochemistry in rat pancreatic samples.Results: Compared with ischemia-reperfusion group,VEGFMCAb can significantly decrease the level of TNF-α,VEGF in serum and apoptosis index.The expression level of Fas,FasL mRNA and protein in I/R group was significant higher than pancreatic tissue in group treating with VEGFMCAb.In I/R group,Fas,FasL immunoreactivity was more intense than in corresponding VEGFMCAb treated tissue.Conclusion: VEGFMCAb can protect pancreas against ischemia-reperfusion injury and can inhibit excessive apoptosis through down-regulation of Fas/FasL.