IN VITRO CULTURE OF BUDS OF GINKGO BILOBA L.
Yan Luo
Abstract
Yan Luo
Abstract
Callus was induced from young stems with axillary buds of Ginkgo biloba L. The best medium for callus induction was MS supplemented with NAA 2 0mg·L -1 , 6 BA0 1mg·L -1 and LH 2 0g·L -1 . Buds could be differentiated from young stems with axillary buds on the medium of 1/2MS+6 BA 2 0mg·L -1 +NAA 0 2mg·L -1 +PP 333 (15% wettable powder)8 0mg·L -1 +AC 0 5g·L -1 . These new formative stems could differentiate roots if they were cultured on the medium of White+“Gen Duo zhuang”2 #(50% IBA.NAA Powder) 2 0mg·L -1 , so that the plantlets were formed. The budding rate is 53 1%. The rootting rate is 90 3%. The surival rate of trasplantation of tube plantlets is 85%.
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Callus was induced from young stems with axillary buds of Ginkgo biloba L. The best medium for callus induction was MS supplemented with NAA 2 0mg·L -1 , 6 BA0 1mg·L -1 and LH 2 0g·L -1 . Buds could be differentiated from young stems with axillary buds on the medium of 1/2MS+6 BA 2 0mg·L -1 +NAA 0 2mg·L -1 +PP 333 (15% wettable powder)8 0mg·L -1 +AC 0 5g·L -1 . These new formative stems could differentiate roots if they were cultured on the medium of White+“Gen Duo zhuang”2 #(50% IBA.NAA Powder) 2 0mg·L -1 , so that the plantlets were formed. The budding rate is 53 1%. The rootting rate is 90 3%. The surival rate of trasplantation of tube plantlets is 85%.
Key concepts: Callus, Ginkgo biloba, Axillary bud, In vitro, Budding, Tissue culture, Horticulture, Botany