2001Journal of Sichuan UniversityRequires access

IN VITRO CULTURE OF BUDS OF GINKGO BILOBA L.

Yan Luo

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Abstract

Callus was induced from young stems with axillary buds of Ginkgo biloba L. The best medium for callus induction was MS supplemented with NAA 2 0mg·L -1 , 6 BA0 1mg·L -1 and LH 2 0g·L -1 . Buds could be differentiated from young stems with axillary buds on the medium of 1/2MS+6 BA 2 0mg·L -1 +NAA 0 2mg·L -1 +PP 333 (15% wettable powder)8 0mg·L -1 +AC 0 5g·L -1 . These new formative stems could differentiate roots if they were cultured on the medium of White+“Gen Duo zhuang”2 #(50% IBA.NAA Powder) 2 0mg·L -1 , so that the plantlets were formed. The budding rate is 53 1%. The rootting rate is 90 3%. The surival rate of trasplantation of tube plantlets is 85%.

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What this paper is about

Callus was induced from young stems with axillary buds of Ginkgo biloba L. The best medium for callus induction was MS supplemented with NAA 2 0mg·L -1 , 6 BA0 1mg·L -1 and LH 2 0g·L -1 . Buds could be differentiated from young stems with axillary buds on the medium of 1/2MS+6 BA 2 0mg·L -1 +NAA 0 2mg·L -1 +PP 333 (15% wettable powder)8 0mg·L -1 +AC 0 5g·L -1 . These new formative stems could differentiate roots if they were cultured on the medium of White+“Gen Duo zhuang”2 #(50% IBA.NAA Powder) 2 0mg·L -1 , so that the plantlets were formed. The budding rate is 53 1%. The rootting rate is 90 3%. The surival rate of trasplantation of tube plantlets is 85%.

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Available abstract

Callus was induced from young stems with axillary buds of Ginkgo biloba L. The best medium for callus induction was MS supplemented with NAA 2 0mg·L -1 , 6 BA0 1mg·L -1 and LH 2 0g·L -1 . Buds could be differentiated from young stems with axillary buds on the medium of 1/2MS+6 BA 2 0mg·L -1 +NAA 0 2mg·L -1 +PP 333 (15% wettable powder)8 0mg·L -1 +AC 0 5g·L -1 . These new formative stems could differentiate roots if they were cultured on the medium of White+“Gen Duo zhuang”2 #(50% IBA.NAA Powder) 2 0mg·L -1 , so that the plantlets were formed. The budding rate is 53 1%. The rootting rate is 90 3%. The surival rate of trasplantation of tube plantlets is 85%.

Key concepts: Callus, Ginkgo biloba, Axillary bud, In vitro, Budding, Tissue culture, Horticulture, Botany

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