Influences of All-trans-retinoic Acid on Proliferation of Thyroid Squamous Cell Carcinoma Cell Line SW579

Objective To observe the influences of all-trans-retinoic acid(ATRA)on proliferation of of thyroid squamous cell carcinoma cell line(SW579)in inducing differentiation in vitro.Methods Treated with different doses of ATRA(final concen- tration of 10~(-7),5×10~(-7),10~(-6),5×10~(-6),10~(-5)mol/L),the cells were observed with an inverted microscopea convert microscope in shape;The the proliferation of the cells was observed with MTT and the cell cycle was measured by flow cytometry.Results Af- ter cells were treated with ATRA,the malignant phenotypes of cells gradually changed to the mature cells.The proliferation index de- creased markedly.The cell cycle was arrested at S stage.Conclusions ATRA can inhibit the proliferation of the cell line SW579 with a dose-dependent manner,and maybe the thyroid cancer cells were induced differentiation in low concentration(10~(-7)mol/L,5×10~(-7)mol/L,10~(-6)mol/L).