Influences of All-trans-retinoic Acid on Apoptosis of Thyroid Squamous Cell Carcinoma Cell Line SW579

Objective To observe the impacts of all-trans-retinoic acid on cell morphologic and apoptosis of thyroid cancer cell line(SW579) in vitro.Methods Treated with different dose of ATRA(final concentration of 10(-7),10(-6),10(-5),2×10(-5),4×10(-5),6×10(-5),8×10(-5),10(-4)mol/L),the cells were observed with a convert microscope in shape.The apoptosis was evaluated by AQ-stained fluorescin,and apoptosis index was measured by flow cytometry.Results SW579 cells treated with ATRA at different concentration underwent apoptosis and presented apoptotic feature: Chromatin condensation,nuclei fragment and apoptotic body formation, flow cytometry analysis showed ATRA increased apoptosis index with the concentration from 10(-7) to 8×10(-5).Conclusions ATRA can induce morphological changes and apoptosis in the thyroid cancer cell line.