Preventive Effect of the Chinese Herbs on the CCl_4-induced Hepatic Fibrosis in Rat

Objective:To study the mechanism of preventive effect of Chinese herbs on the CCl 4 induced hepatic fibrosis in molecular level.Method:After hepatic fibrosis model of rat was established with CCl 4 and Chinese herbs were administrated at the same time of CCl 4 injection,the level of ALRmRNA expression,biochemical and hepatic fibrosis parameters,and pathologic changes were determined.Result:The concentration of ALT,AST,HA,LN,PLD and PCⅢ in serum of hepatic fibrosis rat,and the level of ALR mRNA expression in liver of hepatic fibrosis rat determined by RT PCR increased.After Chinese herbs were administrated by mouth into rat at the same time of CCl 4 injection,the level of ALR mRNA expression in liver cells was higher but the concentration of HA,LN,PLD and PCⅢ in serum were lower than that of the group of hepatic fibrosis model.Conclusion:ALR was involved in liver repair when hepatic fibrosis occurred,and Chinese herbs could prevent hepatic fibrosis by promoting ALR gene expression.