Effect of Seleno-polysaccharide of Eucheuma on Growth and Apoptosis of Hepatoma Carcinoma Cell Strain

[Objective] To investigate the effect of Seleno-polysaccharide on the growth and apoptosis of hepatoma carcinoma cell strain and its mechanism in vitro. [Method] Human hepatoma cells HepG2.2.15 in proliferative phase were divided into three groups:Control group,Seleno-polysaccharid group, and Cisplatin group. Proliferation of tumor cells in each group was tested by MTT method. Cell cycle, cell apoptosis, and expression of Fas gene of tumor cells in each group was detected by flow cytometer.[Results] seleno-polysaccharide inhibited the reduplication of hepatoma carcinoma cell displayed by dose-dependence and time-dependence. The inhibitive rate on proliferation of hepatoma cells by desacetyluvaricin reached up to 97.20%. Parts of the intrinsic mechanism might relate to the blockage of HepG2.2.15 cells at S and G2/M period thus to inhibit tumour cell proliferation, and the induction of HepG2.2.15 apoptosis by promoting expression of Fas gene 14.4% ± 0.11% compared with control group 1.5% ± 0.01%(P 0.05) obviously increased.[Conclusion] Seleno-polysaccharide of eucheuma can inhibit multiplication of hepatoma cell by slowing down cell and inducing cell apoptosis.