Effect of a Monomer Desacetyluvaricin of Annonaceous Acetogenin on Growth and Apoptosis of Hepatoma Carcinoma Cell Strain

Objective To investigate the effect of desacetyluvaricin on the growth and apoptosis of hepatoma carcinoma cell strain and its mechanism in vitro. Method Human hepatoma cells HepG2.2.15 in proliferative phase were divided into three groups: Control group,Desacetyluvaricin group,and Cisplatin group. Proliferation of tumor cells in each group was tested by MTT method. Cell cycle,cell apoptosis,and expression of Fas gene of tumor cells in each group was detected by flow cytometer. Inhibitive effect on tumor cells was compared between desacetyluvaricin and cisplatin Results The inhibitive rate on proliferation of hepatoma cells by desacetyluvaricin reached up to 62.2%. Parts of the intrinsic mechanism might relate to the blockage of HepG2.2.15 cells to go to S period from G1 period thus to inhibit tumour cell proliferation,and the induction of HepG2.2.15 apoptosis by promoting expression of Fas gene. Besides,desacetyluvaricin showed more notable effect on promoting Fas gene expression compared with cisplatin. Conclusion Desacetyluvaricin can inhibit proliferation of hepatoma cells by prolonging G1 period of cell cycle,and inducing cell apoptosis.