Optimization of Total Flavonoids Extract of Pueraria

Objective To optimize the Pueraria optimum extraction technology of total flavonoids. Methods Ethanol was used as the extraction solvent,theextract solvent concentration,extraction time and solid-liquid ratio wereinspectedbyorthogonal L9( 34) design. The sum of the measured characteristic peak areathatwere detected by fingerprinting method,puerarin transfer rate and solids content were used as the evaluation index. The composite score is calculated,and the final score was used to determine the optimum conditions of Pueraria total. Results The main factor thatcan affect the extraction of total flavonoids of Pueraria is solvent concentration,followed by extraction time and solid-liquid ratio. The optimum extraction method is A2B3C2D2,as 70% ethanol,extraction time 3 h,extracted 2 times,materials liquid ratio of 1 ∶8.Conclusion Validation tests showed that the extraction rate of total flavonoids under optimum stability,the extraction rate,the extraction process is reasonablypracticable.