Determination of Seven Pharmacologically Active Phenolic Acids and Its Antioxidant Activity in Buckwheat Honey

Eight Pharmacologically Active Phenolic Acids including protocatechuic, gallic acid, caffeic acid, chlorogenic acid,syingic acid, p-coumaric acid, ferulic acid and ellagic acid were identified in buckwheat honey by high-performance liquidchromatography with electrochemical detector(HPLC-ECD). The results showed that the main phenolic acids were gallic acid,p-coumaric acid, protocatechuic, caffeic acid and ferulic acid. Their concentration in buckwheat honey were 461μg/100g honey,150μg/100g honey, 30μg/100g honey, 79μg/100g honey and 39μg/100g honey respectively. Superoxide radical inhibiting,hydroxyl radical scavenging, 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging and lipid-microsomal peroxidationassay system were used to evaluated the antioxidant activity of buckwheat honey phenolic acids(BWPC). Our results indicatethat BWPC has the capacity to scavenge free radicals and inhibit lipid peroxidation.